Contents
About Nonradioactive Nucleic Acid Detection
Isolation of DNA from Plants
Isolation of High-Molecular-Weight DNA from Animal Cells
Restriction Enzyme Digestion, Gel Electrophoresis, and Vacuum Blotting of DNA to Nylon Membranes
Isolation of Plant RNA
Isolation of Total and PolyA+RNA from Animal Cells
Preparation of RNA Gel Blots
Preparation of RNA Dot Blots
Isolation of Plasmids for the Preparation of Probes
Production of Hybridization Probes by the PCR Utilizing Digoxigenin-Modified Nucleotides
Production of DNA Hybridization Probes with Digoxigenin-Modified Nucleotides by Random Hexanucleotide Priming
Digoxigenin Labeling of RNA Transcripts from Multi- and Single-Locus DNA Minisatellite Probes
Labeling of Double-Stranded DNA Probes with Biotin
Preparation of Horseradish Peroxidase-Labeled Probes
Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP
Labeling of Oligonucleotides with Fluorescein
Hybridization of Digoxigenin-Labeled Probes to Southern Blots and Detection by Chemiluminescence
Southern Blot Hybridization of Digoxigenin-Labeled RNA Minisatellite Probes and Color Detection
Hybridization and Detection of Digoxigenin Probes on RNA Blots
Hybridization of Horseradish Peroxidase-Labeled Probes and Detection by Enhanced Chemiluminescence
Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Enhanced Chemiluminescence
Hybridization of Fluorescein-Labeled Oligonucleotide Probes and Enhanced Chemiluminescence Detection
Preparation of Chromosome Spreads by Root-Tip Meristem Dissection for In Situ Hybridization with Biotin-Labeled Probes
Enzymatic Treatment of Plant Material to Spread Chromosomes for In Situ Hybridization
Use of Biotin-Labeled Probes on Plant Chromosomes
Direct Fluorochrome-Labeled DNA Probes for Direct Fluorescent In Situ Hybridization to Chromosomes
Detection of Digoxigenin-Labeled DNA Probes Hybridized to Plant Chromosomes In Situ
Preparation of Tissue Sections and Slides for mRNA Hybridization
Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes
Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes
PACE (Probe Assay - Chemiluminescence Enhanced): A Magnetic Bead Assay for Noncultural Diagnosis of Gonorrhea
Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format
Analysis of Gene Sequences by Hybridization of PCR-Amplified DNA to Covalently Bound Oligonucleotide Probes: The Reverse Dot Blot Method
RAPD Assay: A Novel Technique for Genetic Diagnostics
Nonradioactive Oligonucleotide Probes for Detecting Products of the Ligase Chain Reaction
Nucleic Acid Sequence-Based Amplification (NASBA)