This book constitutes the refereed proceedings of the 14th International Conference on Principles of Distributed Systems, OPODIS 2010, held in Tozeur, Tunisia, in December 2010. The 32 full papers and 4 brief announcements presented were carefully reviewed and selected from 122 submissions. The papers are organized in topical sections on robots; randomization in distributed algorithms; brief announcements; graph algorithms; fault-tolerance; distributed programming; real-time; shared memory; and concurrency.