This book covers pretty much all of the modern techniques used in biological electron microscopy. It emphasizes the availability of many protocols for both Transmission and Scanning EM. Every chapter has a detailed protocol and a full list of accompanying references, in case the reader wants to know more about a specific topic/technique.It is highly recommended for every student, lab tech, or researcher that wants to have an overview of the existing modern techniques or dare trying a new technique. It's format, with its 600+ pages is not very practical as a lab handbook, but the content information of strict high quality.I have more than 10yrs of experience in biological cryo-EM, and I find this book a very useful addition to my ever growing collection.