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| Ïîèñê ïî óêàçàòåëÿì |
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| Latchman D.S. — Transcription Factors: A practical Approach |
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| Ïðåäìåòíûé óêàçàòåëü |
Promoters, TAATGARAT sequence 1—2
Protein binding to DNA, method 12
Protein concentration, measurement 13
Protein extraction, method using lysogen 140—141
Protein glycosylation, determination of type and site of glycosylation 289
Protein glycosylation, methods of analysis 286—290
Protein phosphorylation in vitro phosphorylation 281—283
Protein phosphorylation, labelling with radioactive phosphorus 270—274
Protein phosphorylation, mapping phosphorylation sites 274—281
Protein phosphorylation, methods of analysis 263—270 283—285
Protein preparation, homogenization of tissue 11—12
Protein preparation, mini-extracts 13—14
Protein preparation, nuclear extracts 10—11
Protein preparation, whole-cell extracts 10—12 13—14
Protein sequences, identification using mass spectrometry 97
Proteins, bacterial synthesis 15
Proteins, measurement of concentration 13
Proteins, method for labelling with ![$[^{32}P]$](/math_tex/25ff13c5ff1f03fbbd33e73c2a7113d382.gif) orthophosphate 271—272
Proteins, recovery and digestion from a gel slice 276—277
Proteolytic clipping band-shift assay, DNA-binding proteins 21—23
Reporter genes for study of activity of transcription factors 196—198
Reporter genes, assays 200—202
Reporter genes, structure 196—198
Retinoblastoma (pRb) protein, phosphorylation 261—262 278—279
Retinoblastoma (pRb) protein, preparation of radioactive 274
Retinoic acid receptors, DNA-binding proteins 21
Reverse-phase chromatography, purification of DNA-binding proteins 111—114
Reverse-phase chromatography, separation of peptides 114
RNA polymerases, for in vitro transcription 188
RNA, method for reverse transcription for PCR analysis 159—160
S300-II, interaction with DNA-binding factor COUP-TF 88—89
S300-II, non-DNA-binding transcription factors 88—89
Saccharomyces cerevisiae, percentage of genes that are transcription factors 178
SDS-polyacrylamide gel, electrophoresis analysis of protein phosphorylation 263—266
SDS-polyacrylamide gel, identification of DNA-binding proteins 111
SDS-polyacrylamide gel, molecular weight determination 76—77 78—80 82—83
SDS-polyacrylamide gel, separation of peptides 114 115
Sequence databases, identification of transcription factors 160 162—163
Serum response factor (SRF), glycosylation 286
Southwestern blotting, for screening cDNA expression libraries 128—130
Sox genes, identification from database 160 162—163
Steroid receptor coactivator protein SRCla 181—182
Steroid receptor coactivator protein SRCla, deletion analysis 185
Steroid receptor coactivator protein SRCla, interaction with oestrogen receptor 207
Steroid receptor coactivator protein SRCla, transactivation domain 202 203
Steroid receptors, DNA-binding proteins 21
Supercoiling, and promoter activity of DNA 226—227
T-tailed vectors, cloning of PCR products 155—156
T4 DNA polymerase, DNA labelling 9—10
T4 polynucleotide kinase, end labelling of DNA 7—8 32—34
TAATGARAT sequence and immediate-early gene transcription 1—2
TAATGARAT sequence, gene promoters 1—2
TAATGARAT sequence, protein binding 1—2 23—24
Target genes, binding activity 176—177
Target genes, molecular cloning 174—177
Target genes, Northern blot analysis 175
Target genes, sequencing 175—176
Target genes, zoo blotting 174—175
Thermal cycle sequencing, method 44—45
Tissues, homogenization for protein extraction 11—12
Transactivation domains, identification using chimeric proteins 202 203
Transactivation domains, steroid receptor coactivator protein (SRCla) 202 203
Transcription and histones 230
Transcription and nucleosomes 230—232
Transcription assays in vitro 63—68 215—217 222—226
Transcription assays, choice of promoters 64
Transcription assays, G-free cassette assay 64—66
Transcription elongation, analysis using mononucleosome templates 231
Transcription elongation, analysis using nucleosome arrays 232
Transcription factor binding sites, binding activity 176
Transcription factor binding sites, methods of isolation 172—174
Transcription factor binding sites, Northern blot analysis 175
Transcription factor binding sites, position on mononucleosome template 243—244
Transcription factor binding sites, sequencing 175—176
Transcription factor binding sites, zoo blotting 174—175
| Transcription factor-DNA complexes, analysis of dissociation 91—92
Transcription factors in vitro transcription and translation 187—189
Transcription factors, analysis of 181—213
Transcription factors, analysis of tertiary structure 84—87
Transcription factors, analysis using gel retardation assay 205—206
Transcription factors, Ap1 23
Transcription factors, binding to nucleosome templates 255—257
Transcription factors, cloning 15—16 123—143 145—164
Transcription factors, cross-linking of dimers 85—86
Transcription factors, cross-linking to DNA 77—82
Transcription factors, dephosphorylation in vitro 266—270
Transcription factors, dimerization 84—86
Transcription factors, DNA-binding specificity 17—19
Transcription factors, E2F family 261—262 264—265
Transcription factors, expression by in vitro transcription and translation 16—17 187—189 195
Transcription factors, expression in bacteria 14—15 189—191 195
Transcription factors, expression in baculovirus 17 194 195
Transcription factors, expression in mammalian cells 192—195
Transcription factors, extraction from nuclei 100—103
Transcription factors, glycosylation 285—290 see
Transcription factors, identification 4—6 75—76 160 162—163
Transcription factors, identification of conserved domains 182
Transcription factors, identification of target genes 165—179
Transcription factors, interactions with other proteins 23—24 92—94 206—212
Transcription factors, interactions with other proteins, ABCD (avidin-biotin complex on DNA) assay 209—211
Transcription factors, interactions with other proteins, analysis using immunoprecipitation 92—94 208—209
Transcription factors, interactions with other proteins, DNA mobility shift assay 209
Transcription factors, interactions with other proteins, GST pull-down assay 206—207
Transcription factors, interactions with other proteins, two-hybrid analysis 211—212
Transcription factors, mapping of functional domains 182—187
Transcription factors, method for expression and purification 168—169
Transcription factors, molecular weight determination 69—83 98
Transcription factors, non-DNA-binding 88—94
Transcription factors, octamer (Oct) family 1—2 20 23—24 261—263 266 268—269 273
Transcription factors, overexpression 193—195
Transcription factors, percentage of genes in yeast 178
Transcription factors, phosphorylation 261—285 290 see
Transcription factors, preparation of deletion mutants 182—185
Transcription factors, preparation of point mutations 185—187
Transcription factors, proving identity 139—142
Transcription factors, purification 17 97—114
Transcription factors, quantity in cells 97
Transcription factors, renaturation from SDS-polyacrylamide gels 82—83
Transcription factors, sources 10—17
Transcription factors, subunit composition 84—87
Transcription factors, use of cDNA clones 15
Transcription factors, use of transfection for study of function 196 197—200 see
Transcription from chromatin 229—259
Transcription in vitro assays 63—68 215—217 222—226
Transcription in vitro, RNA polymerases for 188
Transcription initiation, analysis using nucleosome arrays 231
Transcription, effect of chromatin structure 217 229—232
Transcription, effect of histone acetylation 257 262
Transcription, factor Sp1, glycosylation 286
Transcription, factor Sp1, requirement for zinc 21
Transcriptional machinery, components of 66
Transfection of mammalian cells 196 197—200
Transfection, controls for efficiency 197—198
Transfection, for study of function of transcription factors 196 197—200
Transfection, methods 198—200
Transient transfection, calcium phosphate coprecipitation 199—200
Two-dimensional electrophoresis, analysis of protein phosphorylation 266
Two-hybrid analysis, protein-protein interactions 211—212
U2 small nuclear RNA enhancer, cooperative binding to 7
Ultraviolet light, binding transcription factors to DNA 77—82
Viral component Vmw65 1—2 23
Western blotting, analysis of protein phosphorylation 264—266
Western blotting, identification of transcription factors 75—76
Whole cell extract, method of preparation 195
Whole genome PCR 171
Xenopus, nuclear extracts 233
Yeast, overexpression of cloned transcription factors 194
Zinc-finger proteins, oestrogen receptor 167—168
Zinc-finger proteins, properties 161
Zoo blotting, transcription factor binding sites 174—175
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