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Latchman D.S. — Transcription Factors: A practical Approach
Latchman D.S. — Transcription Factors: A practical Approach



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Íàçâàíèå: Transcription Factors: A practical Approach

Àâòîð: Latchman D.S.

Àííîòàöèÿ:

Univ. College of London, UK. Provides a comprehensive guide to the methods currently available to characterize the function and activity of an individual transcription factor. A new chapter covers the use of in vitro transcription assays. For researchers. Previous edition: c1993. Hardcover, softcover is also available.


ßçûê: en

Ðóáðèêà: Áèîëîãèÿ/

Ñòàòóñ ïðåäìåòíîãî óêàçàòåëÿ: Ãîòîâ óêàçàòåëü ñ íîìåðàìè ñòðàíèö

ed2k: ed2k stats

Ãîä èçäàíèÿ: 1999

Êîëè÷åñòâî ñòðàíèö: 303

Äîáàâëåíà â êàòàëîã: 01.12.2006

Îïåðàöèè: Ïîëîæèòü íà ïîëêó | Ñêîïèðîâàòü ññûëêó äëÿ ôîðóìà | Ñêîïèðîâàòü ID
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Ïðåäìåòíûé óêàçàòåëü
Promoters, TAATGARAT sequence      1—2
Protein binding to DNA, method      12
Protein concentration, measurement      13
Protein extraction, method using lysogen      140—141
Protein glycosylation, determination of type and site of glycosylation      289
Protein glycosylation, methods of analysis      286—290
Protein phosphorylation in vitro phosphorylation      281—283
Protein phosphorylation, labelling with radioactive phosphorus      270—274
Protein phosphorylation, mapping phosphorylation sites      274—281
Protein phosphorylation, methods of analysis      263—270 283—285
Protein preparation, homogenization of tissue      11—12
Protein preparation, mini-extracts      13—14
Protein preparation, nuclear extracts      10—11
Protein preparation, whole-cell extracts      10—12 13—14
Protein sequences, identification using mass spectrometry      97
Proteins, bacterial synthesis      15
Proteins, measurement of concentration      13
Proteins, method for labelling with $[^{32}P]$orthophosphate      271—272
Proteins, recovery and digestion from a gel slice      276—277
Proteolytic clipping band-shift assay, DNA-binding proteins      21—23
Reporter genes for study of activity of transcription factors      196—198
Reporter genes, assays      200—202
Reporter genes, structure      196—198
Retinoblastoma (pRb) protein, phosphorylation      261—262 278—279
Retinoblastoma (pRb) protein, preparation of radioactive      274
Retinoic acid receptors, DNA-binding proteins      21
Reverse-phase chromatography, purification of DNA-binding proteins      111—114
Reverse-phase chromatography, separation of peptides      114
RNA polymerases, for in vitro transcription      188
RNA, method for reverse transcription for PCR analysis      159—160
S300-II, interaction with DNA-binding factor COUP-TF      88—89
S300-II, non-DNA-binding transcription factors      88—89
Saccharomyces cerevisiae, percentage of genes that are transcription factors      178
SDS-polyacrylamide gel, electrophoresis analysis of protein phosphorylation      263—266
SDS-polyacrylamide gel, identification of DNA-binding proteins      111
SDS-polyacrylamide gel, molecular weight determination      76—77 78—80 82—83
SDS-polyacrylamide gel, separation of peptides      114 115
Sequence databases, identification of transcription factors      160 162—163
Serum response factor (SRF), glycosylation      286
Southwestern blotting, for screening cDNA expression libraries      128—130
Sox genes, identification from database      160 162—163
Steroid receptor coactivator protein SRCla      181—182
Steroid receptor coactivator protein SRCla, deletion analysis      185
Steroid receptor coactivator protein SRCla, interaction with oestrogen receptor      207
Steroid receptor coactivator protein SRCla, transactivation domain      202 203
Steroid receptors, DNA-binding proteins      21
Supercoiling, and promoter activity of DNA      226—227
T-tailed vectors, cloning of PCR products      155—156
T4 DNA polymerase, DNA labelling      9—10
T4 polynucleotide kinase, end labelling of DNA      7—8 32—34
TAATGARAT sequence and immediate-early gene transcription      1—2
TAATGARAT sequence, gene promoters      1—2
TAATGARAT sequence, protein binding      1—2 23—24
Target genes, binding activity      176—177
Target genes, molecular cloning      174—177
Target genes, Northern blot analysis      175
Target genes, sequencing      175—176
Target genes, zoo blotting      174—175
Thermal cycle sequencing, method      44—45
Tissues, homogenization for protein extraction      11—12
Transactivation domains, identification using chimeric proteins      202 203
Transactivation domains, steroid receptor coactivator protein (SRCla)      202 203
Transcription and histones      230
Transcription and nucleosomes      230—232
Transcription assays in vitro      63—68 215—217 222—226
Transcription assays, choice of promoters      64
Transcription assays, G-free cassette assay      64—66
Transcription elongation, analysis using mononucleosome templates      231
Transcription elongation, analysis using nucleosome arrays      232
Transcription factor binding sites, binding activity      176
Transcription factor binding sites, methods of isolation      172—174
Transcription factor binding sites, Northern blot analysis      175
Transcription factor binding sites, position on mononucleosome template      243—244
Transcription factor binding sites, sequencing      175—176
Transcription factor binding sites, zoo blotting      174—175
Transcription factor-DNA complexes, analysis of dissociation      91—92
Transcription factors in vitro transcription and translation      187—189
Transcription factors, analysis of      181—213
Transcription factors, analysis of tertiary structure      84—87
Transcription factors, analysis using gel retardation assay      205—206
Transcription factors, Ap1      23
Transcription factors, binding to nucleosome templates      255—257
Transcription factors, cloning      15—16 123—143 145—164
Transcription factors, cross-linking of dimers      85—86
Transcription factors, cross-linking to DNA      77—82
Transcription factors, dephosphorylation in vitro      266—270
Transcription factors, dimerization      84—86
Transcription factors, DNA-binding specificity      17—19
Transcription factors, E2F family      261—262 264—265
Transcription factors, expression by in vitro transcription and translation      16—17 187—189 195
Transcription factors, expression in bacteria      14—15 189—191 195
Transcription factors, expression in baculovirus      17 194 195
Transcription factors, expression in mammalian cells      192—195
Transcription factors, extraction from nuclei      100—103
Transcription factors, glycosylation      285—290 see
Transcription factors, identification      4—6 75—76 160 162—163
Transcription factors, identification of conserved domains      182
Transcription factors, identification of target genes      165—179
Transcription factors, interactions with other proteins      23—24 92—94 206—212
Transcription factors, interactions with other proteins, ABCD (avidin-biotin complex on DNA) assay      209—211
Transcription factors, interactions with other proteins, analysis using immunoprecipitation      92—94 208—209
Transcription factors, interactions with other proteins, DNA mobility shift assay      209
Transcription factors, interactions with other proteins, GST pull-down assay      206—207
Transcription factors, interactions with other proteins, two-hybrid analysis      211—212
Transcription factors, mapping of functional domains      182—187
Transcription factors, method for expression and purification      168—169
Transcription factors, molecular weight determination      69—83 98
Transcription factors, non-DNA-binding      88—94
Transcription factors, octamer (Oct) family      1—2 20 23—24 261—263 266 268—269 273
Transcription factors, overexpression      193—195
Transcription factors, percentage of genes in yeast      178
Transcription factors, phosphorylation      261—285 290 see
Transcription factors, preparation of deletion mutants      182—185
Transcription factors, preparation of point mutations      185—187
Transcription factors, proving identity      139—142
Transcription factors, purification      17 97—114
Transcription factors, quantity in cells      97
Transcription factors, renaturation from SDS-polyacrylamide gels      82—83
Transcription factors, sources      10—17
Transcription factors, subunit composition      84—87
Transcription factors, use of cDNA clones      15
Transcription factors, use of transfection for study of function      196 197—200 see
Transcription from chromatin      229—259
Transcription in vitro assays      63—68 215—217 222—226
Transcription in vitro, RNA polymerases for      188
Transcription initiation, analysis using nucleosome arrays      231
Transcription, effect of chromatin structure      217 229—232
Transcription, effect of histone acetylation      257 262
Transcription, factor Sp1, glycosylation      286
Transcription, factor Sp1, requirement for zinc      21
Transcriptional machinery, components of      66
Transfection of mammalian cells      196 197—200
Transfection, controls for efficiency      197—198
Transfection, for study of function of transcription factors      196 197—200
Transfection, methods      198—200
Transient transfection, calcium phosphate coprecipitation      199—200
Two-dimensional electrophoresis, analysis of protein phosphorylation      266
Two-hybrid analysis, protein-protein interactions      211—212
U2 small nuclear RNA enhancer, cooperative binding to      7
Ultraviolet light, binding transcription factors to DNA      77—82
Viral component Vmw65      1—2 23
Western blotting, analysis of protein phosphorylation      264—266
Western blotting, identification of transcription factors      75—76
Whole cell extract, method of preparation      195
Whole genome PCR      171
Xenopus, nuclear extracts      233
Yeast, overexpression of cloned transcription factors      194
Zinc-finger proteins, oestrogen receptor      167—168
Zinc-finger proteins, properties      161
Zoo blotting, transcription factor binding sites      174—175
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