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Ormerod M.G. Ч Flow Cytometry: A Practical Approach
Ormerod M.G. Ч Flow Cytometry: A Practical Approach

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Ќазвание: Flow Cytometry: A Practical Approach

јвтор: Ormerod M.G.

јннотаци€:

Flow cytometry is a well established, multi-disciplinary technique in standard use in many biological and clinical research laboratories. It also has increasing application in routine clinical work. Understanding the technique and its applications is increasingly important for non-specialists and experienced workers alike. This book provides a practical introduction to the area.


язык: en

–убрика: Ѕиологи€/

—татус предметного указател€: √отов указатель с номерами страниц

ed2k: ed2k stats

»здание: third edition

√од издани€: 2000

 оличество страниц: 276

ƒобавлена в каталог: 23.11.2006

ќперации: ѕоложить на полку | —копировать ссылку дл€ форума | —копировать ID
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ѕредметный указатель
3,3'-dihexyloxacarbocyanine      30 240Ч241
3,3'-dioctadecylindocarbocyanine      31
4-methylumbilleferone      228 229
5-chloromethylfluorescein diacetate (CMFDA)      32
7-Aminoactinomycin D (7-AAD)      28 144 243 245Ч246
Acridine orange      28 29 236
Alexa Fluor Green      27
Allophycocyanin      27
Allophycocyanin-cyanine      7
Allophycocyanin-cyanine, conjugate      27
Alu-polymerase chain reaction      200Ч201
Amplifier linear      17
Amplifier Logarithmic      16Ч17
Analogue to digital converter (ADC)      17 18
Aneuploid      84
Annexin V      243
Anti-coincidence      51
Anti-lymphocyte globulin (ALG), therapy      111Ч113
Anti-thymocyte globulin (ATG), therapy      111Ч113
Antibody, auto-antibody      105 107
Antibody, staining      70Ч71
Antibody, titration      72
Apoptosis      235Ч248
Arc lamp      8
Autofluorescence      69
Basophil      74
Beam splitter      12
BIODIPY      27
Bis-benzimidazole      see УHoechstФ
Bis-carboxyethyl-carboxyfluorescein (BCEF)      228
Blocker bar      6 7
Break-off point      49
Bromodeoxyuridine (BrdUrd)      137 145Ч146 159Ч177 178Ч188
c-myc      137 143
Calcium Crimson      205Ч207
Calcium Green      205Ч207
Calcium ion      31 203Ч221
Calcium ion, calibration      210Ч213
Calcium ion, data analysis      213Ч215
Calcium ion, sorting on the basis of      219Ч220
Calcium Orange      205Ч207
Carboxyfluorescein diacetate      228
Carboxyfluorescein diacetate, succinimidyl ester (CFDA SE)      32
Caspase      32 245
CD (cluster of differentiation)      72
CD, CD14      73 74 75
CD, CD16      73 79
CD, CD19      73 76
CD, CD2      219
CD, CD28      121
CD, CD3      73 76 77 78 79 111 112Ч113 214
CD, CD3, Cytoplasmic      140
CD, CD34      101Ч104 126
CD, CD4      17 19 67 73 77 145 246
CD, CD45      73 74 75 101Ч104 112Ч113 140
CD, CD5      219
CD, CD56      73 79 116
CD, CD68      137
CD, CD69      116
CD, CD79a, cytoplasmic      140
CD, CD8      67 73 78 121 246
Cell activation      116Ч117
Cell count      112
Cell cycle      83Ч84
Cell cycle, analysis      93Ч96
Cell cycle, kinetics      160Ч162
Cell cycle, time $(T_c)$      160 174Ч176
Cell death, associated proteins      153
Cell fixation      82 89 134Ч141
Cell loading      28
Cell loss      160Ч161
Cell lysis      89
Cell permeability      243 244Ч255
Cell permeabilization      134Ч142; see also УElectropermeabilisationФ
Cell permeabilization with detergents      136
Cell proliferation      179Ч188
Cell sorting      249Ч251; see УSorting viabilityФ
Cell stabilization      127
Cell staining      see УAntibody stainingФ
Cell with alcohols      135 136
Cell with aldehydes      135 136
Chlorodeoxyuridine      170
Chloromethyl benzoyl amino tetramethylrhodamine (CMTMR)      32
Chloromethyl-X rhosamine      30
Chromomycin $A_3$      28 29 189Ч191
Chromosome      189Ч201
Chromosome, paints      198Ч201
Chromosome, preparation      192Ч196
Chromosome, sorting      196Ч198
Cisplatin      184Ч185
Clumps of cells      86Ч87
CM-Dil      31 32
Coefficient of variation (cv)      85
Common variable immunodeficiency (CVID)      121Ч122
Compensation      67Ч68
Control isotype      80
Crossmatching      109Ч111
Cy-chrome      see УPhycoerythrin-cyanine5Ф
Cyanine dyes      28Ч30 223
Cyclin      143 145 149Ч151
Cyclosporin A      255Ч256
Cytogram      18 19Ч20
Cytokeratin      143 148Ч149
Cytokine      117Ч122 145Ч146 147
Cytoskeleton      148
Cytotoxicity, cell-mediated      114Ч117
DAPI      see УDiamino-2-phenylindoleФ
Data analysis      18Ч21
Daunomycin      255Ч256
Degenerate oligonucleotide-primed PCR (DOP-PCR)      192 198Ч200
Detector, solid state      15
Diacetoxy-dicyanobenzene (ADB)      228 229
Diamino-2-phenylindole (DAPI)      28
Dichlorodihydrofluorescein      26 32
Dichlorofluorescin      26 32
Dichroic mirror      12
Dicyanohydroquinone (DCH)      228
Dihydrodichlorofluorescein      254
Dihydroethidium      32 254
Dihydrofluorescein      254
Dihydrorhodamine      123 32 254
Dioctadecyloxacarbocyanine      31 32
Diploid      84
Discriminator      16
DNA      28Ч29 81 83Ч97 140
DNA and intracellular antigens      143Ч145 148Ч151
DNA and surface marker      81Ч82
DNA denaturation      167Ч169
DNA histogram      84Ч97 237
DNA histogram, deconvolution      93Ч96
DNA index (DI)      84
Dot plot      18
Drop delay      50 53
Droplet formation      49Ч51
Droplet satellite      49
Electropermeabilisation      251Ч253
Electroporation      251
Energy transfer      3 24Ч25
Enzyme, intracellular      147
Eosinophil      74
Esterase      28 204 249
Ethidium bromide (EB)      28
External quality assurance survey (EQAS)      126 130Ч131
Fibroblast      184Ч185
Filter, bandpass      12
Filter, coloured glass      11
Filter, dichroic      12
Filter, edge      11
Filter, interference      11
Filter, long wavelength pass      12
Filter, optical      11
Filter, short wavelength pass      12
Flow cell      see УFlow chamberФ
Flow chamber      5Ч7
Flow chamber for sorting      7
Flow chamber, analytical      6
Fluidics      3
Fluo-3      31 205Ч206 208Ч209 215Ч217 232
Fluo-4      205Ч206 208Ч209 217
Fluorescein      24 26 27
Fluorescein #di-\beta-D#-galactopyraniside      32
Fluorescein, diacetate (FDA)      26 28 228 249Ч253
Fluorescein, isothiocyanate (FITC)      26
Fluorescence      23Ч25
Fluorescence, quantum efficiency      24
Fluorescence, quenching      24
Fura Red      31 205 215Ч217
Galactosidase      32
Gate, light scatter      75
Gating      19
Glutathione      255 257Ч258
Granulocyte      18 37 74
Granulocyte, reactive antibody      104Ч106
Green fluorescent protein      33
Growth fraction      160
Haemoglobin, fetal      148
Heat shock protein      153
Histogram bivariate      see УCytogramФ
Histogram, frequency      18
HIV      77 80
HLA-B27      126 128
Hoechst 33258      179Ч188 189Ч191
Hoechst 33342      28 29 90 236 243 255
Hormone      148
Hormone, receptor      148
Human platelet antigens (HPA)      107
Hydrodynamic focusing      3
Hydroxyanisole      172Ч173
Hydroxycoumarin      see У4-methylumbilleferoneФ
Hydroxyurea      172Ч173
Immunodeficiency disease      122
Immunoglobulin, cytoplasmic      137
Immunophenotyping      73Ч80
Indo-1      31 204Ч215 217Ч221
Instrument resolution      68Ч70
Instrument sensitivity      68Ч70
Instrument standardization      63Ч68
Interferon-gamma $(IFN-\gamma)$      121Ч122
Interleukin-2      138
Iododeoxyuridine      170Ч171
ISEL      236
Isotype control      76 77 80Ч81 143
JC-1      30 240
Jet-in-air      7
Karyotype      189Ч191
Ki-67      137 151Ч153
Kidney      38Ч39
Labelling index      161 175
lacZ gene      32
Laser      8Ч10
Laser beam shape      10
LDS-751      28 29
Lenses, focussing laser      10
Leucocyte      18 19 36Ч39 64 74 253Ч254
Leukaemia      62
Levey Ч Jennings plot      66
Light scatter      10 18 20 64 75 92Ч93
Lipid      30
Liposome      142
List-mode      20
Lung, rat      40
Lymphoblastoid cell line      185 190
Lymphocyte      18 19 74 182
Lymphocyte, B lymphocyte      76
Lymphocyte, peripheral blood      190
Lymphocyte, reactive antibody      104Ч106
Lymphocyte, T lymphocyte      76 77Ч78 113 246
Lymphoid tissue      38
Lymphoma      191
Magnesium ion      222
MDR-associated protein      255
Membrane permeability      243 244Ч245
Membrane potential      29Ч30 222Ч227
Merocyanine      540 30
microwave      137Ч138
Minimal residual disease1      29
Mithramycin      29
Mitochondrion, membrane potential      30 240Ч242
Mitotic cell antigens      151
MitoTracker Green FM      31 33 242
MitoTracker RED CMXRos      30 242
Mixed lymphocyte reaction      116Ч117
Monobromobimane      257
Monochlorobimane      257
Monocyte      18 74 77
Multi-drug resistance (MDR)      254Ч258
Myeloperoxidase      140
Nile Red      31
NK cells      74 77Ч79
Nucleus      91 93
Octadecyl aminofluorescein      26
Octadecyl fluorescein      32
Oestrogen receptor      138 139
Oncogene-encoded antigens      154
Optics, collection      10
Oregon Green BAPTA      205 207
Oxidative burst      253Ч254
Oxidative species      32 253
Oxonol dyes      29 223
p-glycoprotein pump      255
p53      139 143
Paraffin-embedded tissue      91Ч93 139
PerCP      see УPeridinin chlorophyllФ
Peridinin chlorophyll (PerCP)      27
Periodate-lysine-formaldehyde (PLP)      137
Peripheral blood      35Ч45
PH calibration      232
PH cytoplasmic      32 227Ч232
Phase angle      52
Phase gating      52
Phosphatidyl serine (PS)      243Ч244
Photomultiplier      13 15
Phycoeiythrin-cyanine5 (Cy-chrome)      27
Phycoeiythrin-cyanine7      27
Phycoeiythrin-TexasRed (ECD)      27
Phycoerythrin      27
PIN diode      13 15
PKH26      31 32 114Ч116
pl05      136 139 151
Platelets      36
Platelets, reactive antibodies      107Ч108
Ploidy      84; see also УDiploidФ УAneuploidФ
Polymerase chain reaction (PCR)      191Ч192
Population assignment      72
Potential doubling time $(T_{pot})$      161 174Ч176
Preamplifier      16
Progesterone receptor      138 139
Proliferating nuclear antigen (PCNA)      136 138Ч139 143 153
Propidium iodide (PI)      28Ч29 87Ч93 144 238Ч244 249Ч253
Pulse processing      16
Pyronin Y      28
Quality control      61 85 125Ч131
Quin2      204
Radiation, gamma      184Ч185
Ratiometric analysis      204 213Ч216
Relative movement      174Ч176
Reporter molecule      33
Reticulocyte      99Ч101
Rhodamine 110      31 33
Rhodamine 123      30 240
1 2
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