Crystalline lens consists of epithelial cells, fiber cells, and a capsule. They originate from lens epithelial cells. Epithelial cells differentiate into fiber cells and produce collagen, which is the major compound of the capsule. The lens epithelial cells maintain normal physiology and homeostasis of the lens, so the cultures of human lens epithelial (HLE) cells provide important information concerning the role of epithelium in normal and cataract formation