Balkwill F. (ed.) — Cytokine Molecular Biology :: Электронная библиотека попечительского совета мехмата МГУ

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Balkwill F. (ed.) — Cytokine Molecular Biology

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Название: Cytokine Molecular Biology

Автор: Balkwill F. (ed.)

Аннотация:

Cytokine Molecular Biology concentrates on molecular biology techniques for the study of cytokines, cytokine receptors, and cytokine driven processes. Updated topics from the previous edition are: the cloning and expressing cytokine genes;; the detection of cytokine mRNA; receptor binding studies; the PC-specific phosphslipase C and sphingomylinases. In addition, new topics covered are the purification, sequencing, and synthesis of cytokines; studying cytokine gene polymorphisms; the use of proteomics in cytokine research; and the Jak/STAT and MAPK signalling pathways. Written by experts in the field, Cytokine Molecular Biology and Cytokine Cellular Biology form a comprehensive and essential guide to cytokine research.

Язык:

Рубрика: Биология/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Год издания: 2001

Количество страниц: 201

Добавлена в каталог: 20.11.2006

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID

Предметный указатель

5' Nuclease assay      36—38
Acidic Smase      150
Allelic discrimination methods      25—29
Amino-acid sequence analysis      81—84
Antibodies to study cell, signalling      107—108 112—115 119 122
Antisense asymmetric RNA probes      63—64
Association studies for disease-causing alleles      40—43
Auto-Kinase assay      116—117
Autographa californica MNPV vector      11
Automated protein sequences      81—82
Autoradiography in situ hybridisation      70—71
BLAST program      9
Blastocyst injection      176—180
Bradford assay      110
Case control studies for gene polymorphism      23—25 40—41
cDNA assays      48
cDNA libraries      1—20
cDNA synthesis      2—3 16 58—59
Cell signalling      see receptor signalling
Ceramide      134 136—137
Charring densitometry      146—148
Chemical synthesis of chemokines      see peptide synthesis
Chemokines - purification      73—88
Chimaeric mice      155—188
CHO cells      11
Cloning of cytokine genes      1—20
Cloning of cytokine genes, IFN- $\alpha / \beta$ receptors      100—101
Cloning of cytokine genes, IFN-7 receptor      98—100
Conditional gene targeting      180—186
Contamination in PCR-based techniques      38—39
COS cells      10
Covalent crosslinking of ligand to receptor      96—97
CPG      75—79
Cre recombinase      180—186
Cre-expressing transgenic lines      183—186
Crosslinking of ligand to receptor      96—97
Cysteine residue modification      84—85
Cytosolic extracts      126—127
Dag      134 136—137
DNase treatment of RNA      51—53
Egg transfer for transgenic mice      160—161
Electrotransformation of E. coli      5
Embryonic stem cells      153 88
EMSA      125—130
ES cells      see embryonic stem cells
EST databases      9
Expression of cytokine genes      1—20
Expression of cytokine genes, bacterial systems      5 7 14—16
Expression of cytokine genes, insect cells      11
Expression of cytokine genes, lower eukaryotes      12—14
Expression of cytokine genes, mammalian cells      7
Expression of cytokine genes, permanent expression      10—11
Expression of cytokine genes, transient expression      10
Expression vectors      4 10—16
Fluorescence-based allelic, discrimination      35—36
G proteins      105—107
GAPDH control      52
Gel retardation assay      see EMSA
Genetic variation of cytokine loci      see polymorphisms of cytokine genes
Genomic DNA preparation      27—28
Haplotype relative-risk analysis      41
HEK293T cells      10
Immunoprecipitation      114—115
In situ hybridisation to cytokine mRNA      62—72
In situ hybridisation to cytokine mRNA, antisense probes      65
In situ hybridisation to cytokine mRNA, autoradiography      70—71
In situ hybridisation to cytokine mRNA, controls      64—65
In situ hybridisation to cytokine mRNA, counterstaining      71—72
In situ hybridisation to cytokine mRNA, dark field microscopy      71—72
In situ hybridisation to cytokine mRNA, DNA probes      67—68
In situ hybridisation to cytokine mRNA, hybridisation      68—69
In situ hybridisation to cytokine mRNA, Interpretation      71—72
In situ hybridisation to cytokine mRNA, posthybridisation      69—70
In situ hybridisation to cytokine mRNA, prehybridisation      63 66—67
In situ hybridisation to cytokine mRNA, probe choice      63—64
In situ hybridisation to cytokine mRNA, probe labelling      67—68
In situ hybridisation to cytokine mRNA, sample handling      63
In situ hybridisation to cytokine mRNA, sample preparation      66—67
In situ hybridisation to cytokine mRNA, sense probes      65
In situ hybridisation to cytokine mRNA, slide preparation      65
In vitro kinase assay      115—116 112—113
Inflammatory diseases      21—46
Interferon- $\beta$ cloning      14
Interferon- $\gamma$ , control for northern blotting      52
Interferon- $\gamma$ , expression      11
Interleukin-2(IL-2), cloning      13 14
Interleukin-2(IL-2), control for northern blotting      52
Interleukin-4(IL-4) cloning      14
Interleukin-5(IL-5) expression      13
Interleukin-6(IL-6), control for northern blotting      52
Interleukin-6(IL-6), expression      11 13—14
Interleukin-l(IL-l), control for northern blotting      52
Interleukin-l(IL-l), genetic polymoprohisms      21—46
Internal sequence analysis      83—84
Jak kinases      105 114—115
Knockout mice      166—188
Knockout mice, blastocyst injection      176—180
Knockout mice, chimaeric mice generation      179
Knockout mice, conditional gene targeting      180—186
Knockout mice, electroporation of DNA      171—172
Knockout mice, embryonic stem (ES) cells      167—168
Knockout mice, extraction of DNA      175—176
Knockout mice, feeder cell layers      168—169
Knockout mice, freezing ESC clones      173—174
Knockout mice, gene targeting ES      169—171
Knockout mice, germline chimeras      176—180
Knockout mice, lacZ assays      184—186
Knockout mice, picking and expansion of ESC cells      172—173
Knockout mice, storage and recovery of ESC cells      174—175
LacZ activity      184—188
Lipid extraction      140—141
Lipidkinases      105—107
Lysis of cells      109—111
MAPK pathway activity      119—123
Metabolic cell labelling      139—140
MHC and immune response      21 23
Microinjection      156—160
MlP- $1\alpha/ \beta$       73
Neutral Smase      149
Non-parametric linkage analysis      42—43
Northern analysis of cytokine mRNA      52—58
Northern analysis of cytokine mRNA, blotting      54—55
Northern analysis of cytokine mRNA, electrophoresis      53—54

Northern analysis of cytokine mRNA, handling RNA      48—49
Northern analysis of cytokine mRNA, hybridisation      58—58
Northern analysis of cytokine mRNA, isolation of RNA      49—51
Northern analysis of cytokine mRNA, preparation of RNA      48—49
Northern analysis of cytokine mRNA, probe labelling      55—56
Nuclear protein extracts      126—127
Oligonucleotide screening      8
Oocyte expression of cytokines      5—6
Oocyte recovery      158—159
PC-specific phosphlipase C      133—153
PCR for hybridisation probes      9
PCR reaction      59—61
PCR-grade DNA preparation      26—27
PCR-RFLPgenotyping      29—34
Peptide synthesis      85—88
Peptide synthesis, solid phase      87—88
Percill preparation, phospholipase D      133—154
Phosphatidyl choline      133—154
Phosphatidylcholine-specific, phospholipase C      133—154
Phosphotyrosine western blotting      112—115
Pkhiapastoris      13
Polymerase chain reaction      28—29
Polymerase chain reaction for cytokine mRNA      58—61
Polymerase chain reaction for cytokine mRNA, cDNA synthesis      58—59
Polymerase chain reaction for cytokine mRNA, cycle parameters      50—61
Polymerase chain reaction for cytokine mRNA, primers      60
Polymerase chain reaction for cytokine mRNA, reaction      59—60
Polymerase chain reaction for cytokine mRNA, verification      61
Polymorphisms of cytokine genes      21—46
Polymorphisms of cytokine genes, case control studies for TNF and IL-1 gene polymophism      23—25
Polymorphisms of cytokine genes, genetic variation at IL-1 and TNF loci      21—23
Polymorphisms of cytokine genes, methods for allelic discrimination in IL-1 and TNF gene clusters      25—26
Polymorphisms of cytokine genes, quality controls      38—39
Polymorphisms of cytokine genes, screening      30—36
Polymorphisms of cytokine genes, study design      39—43
Primers for cytokine RT-PCR      60
Protein fragmentation for internal sequence analysis      83—84
Protein purification      73—88
Purification of cytokines and chemokines      73—88
Purification of cytokines and chemokines, adsorption to CPG      75—80
Purification of cytokines and chemokines, adsorption to silicic acid      79—81
Purification of cytokines and chemokines, purification of chemokines from natural sources      75—81
Purification of cytokines and chemokines, sequence analysis      81—85
Radiolabelling, consensus oligonucleotides      127—129
Radiolabelling, cytokines      90—92
Radiolabelling, probes      55—56
RANTES      73
Receptor signalling      105—153
Receptor signalling, acidic Smase      150
Receptor signalling, auto-kinase assay      116—117
Receptor signalling, ceramide      134 136—137
Receptor signalling, charring densitometry      146—148
Receptor signalling, cytostolic extracts      126—127
Receptor signalling, DAG      134 138—143
Receptor signalling, determination of cell lysate protein concentration      111
Receptor signalling, EMSA      125—126 129—130
Receptor signalling, inhibitors      108
Receptor signalling, JAK kinase activity      114—115
Receptor signalling, lipid extraction      140—141
Receptor signalling, MAPK pathway activity      119—120
Receptor signalling, metabolic cell labelling      139—140
Receptor signalling, neutral Smase      149
Receptor signalling, nuclear protein extracts      126—127
Receptor signalling, PC-specific phospholipase C      133—153
Receptor signalling, phosphotyrosine western blotting      112—114
Receptor signalling, preparation of cells for signalling studies      108—111
Receptor signalling, radiolabelling consensus oligonucleotides      127—129
Receptor signalling, serine-threonine kinases      118—119
Receptor signalling, sphingomyelinases      133—153
Receptor signalling, Src kinase assays      118
Receptor signalling, thin-layer chromatography for detection of DAG      141—143
Receptor signalling, thin-layer chromatography for detection of Pchol      143—145
Receptor signalling, transcription factor assay      123—124
Receptor signalling, tyrosine phosphorylation and tyrosine kinases      111—114
Receptor-binding studies      89—104
Receptor-binding studies, binding of ligand      93—95
Receptor-binding studies, covalent crosslinking      96—97
Receptor-binding studies, radiolabelling interferon      91—92
Receptor-binding studies, scatchard analysis      94
Ribonuclease protection assay      61—62
RNA      1—4 47—72
RNA, conversion to cDNA      2—3
RNA, in situ hybridisation      62—72
RNA, Northern blotting      51—57
RNA, preparation      1—2 48—52
RNA, ribonuclease protection assay      61—62
RT-PCR for cytokine mRNA      58—61
Saccharomyces cerevisiae secretion system      12
Scatchard analysis      94
Serine/threonine kinases      118—119
Signalling from cytokine receptors      see receptor Signalling
Silicic acid      75—76
Slot blots of tail DNA      163—164
Solid-phase peptide synthesis      87—88
Sphingomyelinases      133—153
Spodopterafrugiperda-derived cell lines      11
Src kinases      105 118
STAT transcription factors      124—130
Syk kinases      105
TagMan allelic discrimination      36—38
Tail DNA preparation      162—163
Tec kinases      105
Thin layer chromatography      141—145
TRADD      105
TRAFs      105
Transcription factors      105—107 123—130
Transgenic mice      155—188
Transgenic mice, animals      155—156
Transgenic mice, egg transfer      160—161
Transgenic mice, integration / expression transgenes      161—162
Transgenic mice, micromjection      159—160
Transgenic mice, PCR screening      165
Transgenic mice, pronuclear injection of DNA      157—158
Transgenic mice, purification of DNA      156—157
Transgenic mice, recovery of oocytes      158—159
Transgenic mice, slot blots of tail DNA      163—164
Transgenic mice, tail DNA preparation      162—163
Transmission/disequilibrium tests (TDT)      41—43
Tumour necrosis factors (TNFs), genetic polymorphism      21—46
Tumour necrosis factors (TNFs), signalling pathways      105—132
Tyrosine kinases      105—131
Tyrosine phosphorylation      105—131
Western blotting      112—114 119—123
Xenopus laevis oocytes      5—6 90 94—95

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