Describes methods for the analytical and preparative isolation of specific populations of biological cells. Covers basic methods such as lytic removal of cell subsets, and details new developments in methods using endogenous physical cell properties such as cell size and cell density. Describes more specific cell separation methods based on monoclonal antibodies against cell surface markers, set out basic technical principles of the methods, and discusses typical examples with performance data, looking at applications in basic research and medicine. Includes an appendix of cell properties for cell separations and CD antigens. For those involved in clinical cellular therapy for transplantation, cancer, and AIDS, and in gene therapy.