van der Loos C.M. — Immunoenzyme Multiple Staining Methods :: Электронная библиотека попечительского совета мехмата МГУ

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van der Loos C.M. — Immunoenzyme Multiple Staining Methods

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Название: Immunoenzyme Multiple Staining Methods

Автор: van der Loos C.M.

Аннотация:

This book describes double and triple immunoenzyme staining methods which can be performed with commercially available reagents, and provides generic protocols which can be easily adapted to many different applications. It provides advice for which stains to select, how they can be applied, and invaluable trouble-shooting advice to guide a new user in the field." "Immunoenzyme Multiple Staining Methods is a simple and essential guide through the many different multiple staining methods and colour combinations available, which can often be viewed as complex and confusing. It is an invaluable practical companion for cell biologists, pathologists and histologists new to multiple staining, and is also a useful reference for more experienced workers.

Язык:

Рубрика: Биология/

Статус предметного указателя: Готов указатель с номерами страниц

ed2k: ed2k stats

Год издания: 1999

Количество страниц: 118

Добавлена в каталог: 16.11.2006

Операции: Положить на полку | Скопировать ссылку для форума | Скопировать ID

Предметный указатель

$\beta$ -Galactosidase      3 43—45 47 49 53 63 97 95—96 100
$\beta$ -Galactosidase, Bluo-GAL      43—45 47 96
$\beta$ -Galactosidase, X-GAL      12 43—45 49 53 95 97
Adobe Photoshop      49
Alkaline phosphatase      3 43—48 53 57 63 83 94—95 97
Alkaline phosphatase, endogenous alkaline phosphatase activity      52
Alkaline phosphatase, Fast Blue      43—47 53 65 94
Alkaline phosphatase, Fast Red      12 43—47 83 90 94 97
Alkaline phosphatase, NBT/BCIP      12 43—46 61 90 95 97
Alkaline phosphatase, New Fuchsin      43—45 47 49 95 97
Alkaline phosphatase, Vector Red      43—45 47—48 97
Aminoethylcarbazole      12 43—47 60—61 65 93 97
Animal Research Kit (ARK)      41 103
Antibody tracers, enzymes      3
Antibody tracers, fluorochromes      13 57 84 87—88 103
Antibody, primary, dilution      43
Antibody, primary, Ig isotype and IgG subclass      14—15 38 63 86
Antibody, primary, replacement      39
Antibody, primary, two IgGl      39 41
Antigen retrieval      2—3 12 43 60—61 77 78 80 90
APAAP      5 35 42 52 86 88
Apoptosis      8 59 62
Biotin label      13 34 41 88 103
Biotin label, endogenous biotin      42—43 52
Biotin-(strept)avidin      4 42—43 65 87
Bluo-GAL      43—45 47 96
Card      4
Chromogens, accuracy      43—44 46—48 57
Chromogens, mounting media      46—49
Chromogens, order of development      53 64—65
Chromogens, selection      51—53
Chromogens, sensitivity/efficiency      42—43 53 94
Chromogens, shielding      11—12 53 59
Co-localization, definition      7—8
Co-localization, mixed-colours      8 38 46—48 61
Co-localization, restrictions      57
Co-localization, triple staining      63—64
Colour combinations      36 44—49 51—53 61—62 64—65 103
Conjugation      3 9
Conjugation, $\beta$ -galactosidase      101
Conjugation, biotin      41 99
Conjugation, digoxigenin      99
Conjugation, FITC      41 100
Cryostat sections      2 37 52 79
Detection systems      3 5
Detection systems, sensitivity/efficiency      14—15 42 57
Diaminobenzidin      11—12 43—46 52—53 59 61—62 65 83 90 93 97
Digoxigenin      42 61 99
Double staining, aims      8
Double staining, concepts      11—15 34—36
Double staining, controls      38
Double staining, demands      9
Double staining, imaging      49
Double staining, multi-step      15 34—36 45 87—88
Double staining, protocols      83—88
Double staining, sequential      11—12 39 45 59—61 83 103
Double staining, simultaneous      13—15 45 84—87
Double staining, strategy      37—38
Endogenous, alkaline phosphatase activity      52
Endogenous, biotin      42—43 52

Endogenous, immunoglobulins      41 104
Endogenous, peroxidase activity      47 52 79
Endogenous, tissue pigments      52
EnVision      4 12 14—15 35 41 42 52 83 88 90
Epi-polarization microscopy      5 43 48 89
EPOS      4 12 34 36 42 52 84 88 103
Fast Blue      43—47 53 65 94
Fast Red      12 43—47 83 90 94 97
Fixation, coagulant      2—3
Fixation, cross-linking      2—3
Fluorescent alkaline phosphatase      5 48 57
Formalin      2—3 52 79
Immunoelectron microscopy      9
Immunofluorescence      1 5—6 8 57 64
Immunogold-silver      4—5 11 43 48 57 89
Immunohistochemistry, definition      1
Immunohistochemistry, history      1—6
Immunohistochemistry, single staining      37 79 80
In situ hybridization      6 8 60 61 64
Methacarn      2—3 78 80
Multiple staining, general      6
Multiple staining, getting started      103
Multiple staining, strategy      37—38
NBT/BCIP      12 43—46 61 90 95 97
New Fuchsin      43—45 47 49 95 97
Nuclear counterstain, haematoxylin      47 48 65
Nuclear counterstain, methylgreen      46 62 78
Paraffin sections      2—3 37 52 80
Paraffin sections, antigen retrieval      2—3 12 43 60 61 77 78 80 90
Paraffin sections, pretreatments      1—3 80
Peroxidase      1 3 11 43—47 52—53 60 63 83 93—94 97
Peroxidase, aminoethylcarbazole      12 43—47 53 60—61 65 93 97
Peroxidase, diaminobenzidin      11—12 43—46 52—53 59 61—62 65 83 90 93 97
Peroxidase, endogenous peroxidase activity      47 52 79
Peroxidase, tetramethylbenzidin      43—45 47 94 97
Selection of, colour combinations      36 44—49 51 53 103
Selection of, detection system      41—44
Selection of, double staining concept      39—41
Sensitivity/efficiency, chromogens      42—43
Sensitivity/efficiency, detection systems      42
Sensitivity/efficiency, too low      14 15 52—53 57
Serial sections      7
Silver enhancement      4—5 48 89 97
Single staining      37 79 80
Substrates      see chromogens
Tetramethylbenzidin      43—45 47 94 97
Tissue sections, cryostat      2 37 52 79
Tissue sections, fixation      1—2
Tissue sections, paraffin      2—3 37 52 80
Tissue sections, pretreatments      1—3 80
Tissue sections, storage      79
Triple staining      6 38 63—65
Trouble shooting      51 54—56 104—105
TUNEL      8 59—62 77 78 90
Ultra-small gold      4—5 48
Ultra-small gold, epi-polarization      5 43 48
Ultra-small gold, silver enhancement      4—5 48 89 97
Vector Red      43—45
X-GAL      12 43—45 47 49 53 60 65 95 97
Zamboni      2 78 79

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