This book constitutes the refereed proceedings of the 8th International Semantic Web Conference, ISWC 2009, held in Chantilly, VA, USA, during October 25-29, 2009. The volume contains 43 revised full research papers selected from a total of 250 submissions; 15 papers out of 59 submissions to the semantic Web in-use track, and 7 papers and 12 posters accepted out of 19 submissions to the doctorial consortium. The topics covered in the research track are ontology engineering; data management; software and service engineering; non-standard reasoning with ontologies; semantic retrieval; OWL; ontology alignment; description logics; user interfaces; Web data and knowledge; semantic Web services; semantic social networks; and rules and relatedness. The semantic Web in-use track covers knowledge management; business applications; applications from home to space; and services and infrastructure.