This book constitutes the thoroughly refereed post-conference proceedings of the 14th International Meeting on DNA Computing, DNA 14, held in Prague, Czech Republic, in June 2008. The 15 revised full papers presented were carefully reviewed and selected from 59 submissions. Their topics include theoretical models of biomolecular computing, demonstrations of biomolecular computing processes, self-assembly systems, DNA nanostructures and nanomachines, biotechnological and other applications of DNA computing, and other related themes.