This book constitutes the thoroughly refereed post-proceedings of the Second International Workshop on Applications of Graph Transformations with Industrial Relevance, AGTIVE 2003, held in Charlotesville, Virginia, USA in September/October 2003. The 27 revised full papers and 11 revised demo papers presented together with 2 invited papers and 5 workshop reports were carefully selected during iterated rounds of reviewing and revision. The papers are organized in topical sections on Web applications; data structures and data bases; engineering applications; agent-oriented and functional programs and distribution; object- and aspect-oriented systems; natural languages: processing and structuring; reengineering; reuse and integration; modeling languages; bioinformatics; and multimedia, picture, and visual languages.