Lorette Javois’ timely new 2nd edition of Immunocytochemistry Methods revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization. From Reviews of the First Edition: "…a useful, sometimes beautiful compilation of protocols and methods…" — FEBS Letters "…an extremely useful ‘vade mecum’ of comprehensive, detailed recipes, good advice and helpful information, spiral bound in a handy size for laboratory use." — Society for General Microbiology Quarterly