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| Ïîèñê ïî óêàçàòåëÿì |
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| Polak J.M., Van Noorden S. — Introduction to Immunocytochemistry |
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| Ïðåäìåòíûé óêàçàòåëü |
 -D-Galactosidase 2 27 37 39 122
-D-Galactosidase in double immunoenzymatic stain 37 39
-D-Galactosidase, development 122
3-Amino-9-ethylcarbazole (AEC) 25 118—119
3-Amino-9-ethylcarbazole (AEC) in multiple staining 36—39 (Plates 5 14)
4-Chloro-1-naphthol 25
4-Chloro-1-naphthol in multiple staining 119
Absorption control for specificity 133—134
Adherence of sections to slides 16 110—111
Adherence of sections to slides, poly-L-lysine 16 110—111
Adherence of sections to slides, silane (APES) 16 111
Alkaline phosphatase anti-alkaline phosphatase (APAAP) 51
Alkaline phosphatase anti-alkaline phosphatase (APAAP) in multiple staining 37 39
Alkaline phosphatase anti-alkaline phosphatase (APAAP), build-up method 63
Alkaline phosphatase in multiple staining 36—39 (Plates 12—14)
Alkaline phosphatase, blocking endogenous enzyme 26
Alkaline phosphatase, development 119—121
Alkaline phosphatase, development, blue end-product 120
Alkaline phosphatase, development, blue-brown end-product 120—121
Alkaline phosphatase, development, red end-product 36 38 120
Alkaline phosphatase, mechanism of action 26
Antibody, application to preparations 45—46
Antibody, characteristics 9—10 44—45
Antibody, characteristics, affinity 9
Antibody, characteristics, avidity 9
Antibody, characteristics, diluent 42 110
Antibody, characteristics, dilution 10 42—44
Antibody, characteristics, dilution in relation to background 36 38 8) 42—44
Antibody, characteristics, dilution in relation to sensitivity 37 39 10) 44
Antibody, characteristics, dilution in relation to temperature 43
Antibody, characteristics, dilution in relation to titration 43
Antibody, characteristics, dilution in relation to titre 10
Antibody, characteristics, IgG reaction with antigen 44—45
Antibody, monoclonal 8
Antibody, production 5—7
Antibody, production, immunization 5—6
Antibody, region-specific 6—7 61
Antibody, storage 110
Antibody, testing, blotting methods 6 93—94
Antibody, testing, ELISA 6 92—93
Antibody, testing, immunocytochemical titration 6 36 38 8) 42—44
Antibody, testing, RIA 6 92
Antigen retrieval 16—20
Antigen retrieval, enzyme pretreatment 17—18 114—115
Antigen retrieval, enzyme pretreatment, chymotrypsin 18
Antigen retrieval, enzyme pretreatment, neuraminidase 115
Antigen retrieval, enzyme pretreatment, pepsin 115
Antigen retrieval, enzyme pretreatment, protease XXTV 18 114
Antigen retrieval, enzyme pretreatment, trypsin 18 114
Antigen retrieval, heat-mediated 19—20 116—117
Antigen retrieval, heat-mediated, microwave method 36 38 116
Antigen retrieval, washing 17
Applications of immunocytochemistry 95—100
Applications of immunocytochemistry, pathological diagnosis 95—96
Applications of immunocytochemistry, quantification 97—100
Applications of immunocytochemistry, quantification, confocal microscopy 37 39 16) 98
Applications of immunocytochemistry, quantification, FACS 98 99
Applications of immunocytochemistry, quantification, flow cytometry 98—99
Applications of immunocytochemistry, quantification, supra-optimal dilution 99—100
Applications of immunocytochemistry, research 97
Avidin—biotin methods 51—54 125—128
Avidin—biotin methods, ABC versus PAP 37 39 10)
Background staining see Non-specific staining
Biotin 2 29
Biotin in ABC methods 51—54
Biotin, blocking endogenous biotin 52—53 112
buffers 41
Buffers, PBS 41 109
Buffers, TBS 41 109
Colloidal gold, label for electron microscopical, immunocytochemistry 85—86 133—135
Colloidal gold, label for electron microscopical, multiple labelling 87—88
Colloidal gold, label for electron microscopical, quantification 97
Colloidal gold, label for electron microscopical, with Protein A or G 86
Colloidal gold, label for light microscopical, dark-field illumination 106
Colloidal gold, label for light microscopical, epipolar illumination 106
Colloidal gold, label for light microscopical, immunocytochemistry 2 20 21 27—28
Colloidal gold, label for light microscopical, labelling antibodies 21 27
Colloidal gold, label for light microscopical, silver intensification 27 128—130
Colloidal gold, label for light microscopical, uses 28
Controls 55—62 96 133—134
Controls, absorption with antigen 59 60 133—134
Controls, absorption with serum 58
Controls, absorption with tissue powder 60
Controls, experimental 61—62
Controls, negative 61 96
Controls, positive 61 96
Controls, testing for non-specific binding 55—58
Controls, testing for non-specific binding, by primary antibody 55—57
Controls, testing for non-specific binding, by second or third reagents 57
Controls, testing for non-specific binding, due to basic proteins in tissue 58
Controls, testing for unwanted specific, binding 58—60
Controls, testing for unwanted specific, due to cross-reactivity with related antigens 58—59
Controls, testing for unwanted specific, due to unknown tissue antigens 57
Counterstains to mask autofluorescence 31
Counterstains, fluorescent 21—22
Definition of immunocytochemistry 1
Detergent for permeabilization 14
Detergent to prevent non-specific binding 41 42
Diaminobenzidine (DAB) 24—25
Diaminobenzidine (DAB) for electron microscopical immunoperoxidase 84
Diaminobenzidine (DAB), intensification of reaction product 66—70
Diaminobenzidine (DAB), intensification of reaction product, cobalt 123
Diaminobenzidine (DAB), intensification of reaction product, copper sulphate 37 39 122
Diaminobenzidine (DAB), intensification of reaction product, gold chloride 123
Diaminobenzidine (DAB), intensification of reaction product, imidazole 123
Diaminobenzidine (DAB), intensification of reaction product, nickel 37 39 14) 124
Diaminobenzidine (DAB), mechanism of reaction 25
Diaminobenzidine (DAB), re-use 118
Diaminobenzidine (DAB), safety 24—25 118
Dot blots, as antibody test 94
Double immunoenzymatic staining 37 39 78 131
Double immunofluorescence staining iv (front cover illustration) 76 77 79 105
Electron microscopical, fixation 81—82
Electron microscopical, fixation, glutaraldehyde 82
Electron microscopical, fixation, paraformaldehyde 82
Electron microscopical, immunolabelling 81—88 132—133
Electron microscopical, labels 84—86
Electron microscopical, labels, colloidal gold 85—86
Electron microscopical, labels, ferritin 84
Electron microscopical, labels, peroxidase 84—85
Electron microscopical, multiple labelling 87—88
Electron microscopical, pretreatment 87
Electron microscopical, principles 81
Electron microscopical, procedure 86 132—133
Electron microscopical, resins 82—83
ELISA, as antibody test 92—93
Endogenous enzyme blocking alkaline phosphatase 26
Endogenous enzyme blocking peroxidase 24 112—113
Endogenous enzyme blocking peroxidase, frozen sections and whole cells 113
Endogenous enzyme blocking peroxidase, paraffin sections 112—113
Enhancement of standard methods, build-up methods 63—65
Enhancement of standard methods, build-up methods, ABC 64—65
Enhancement of standard methods, build-up methods, APAAP 63
Enhancement of standard methods, build-up methods, indirect 63—64
Enhancement of standard methods, build-up methods, PAP 63—64
Enhancement of standard methods, during reaction 67 123—125
Enhancement of standard methods, intensification of, peroxidase/DAB/ product 66—70 122—124
Enhancement of standard methods, post-reaction 66—67 122—123
Enhancement of standard methods, tyramine signal amplification 67—70
Enzyme labels 2 20 23—27
Enzyme labels, -D-galactosidase 2 27 37 39
Enzyme labels, -D-galactosidase, development 122
Enzyme labels, alkaline phosphatase, blocking endogenous enzyme 26
Enzyme labels, alkaline phosphatase, development 119—121
Enzyme labels, alkaline phosphatase, in multiple staining 36—39 (Plates 12—14)
Enzyme labels, alkaline phosphatase, mechanism of action 26
Enzyme labels, glucose oxidase 2 26—27
Enzyme labels, glucose oxidase, development 122
Enzyme labels, peroxidase, blocking endogenous enzyme 24
Enzyme labels, peroxidase, development 24—26 66—70 117—119 122—125
| fixation 11—15
Fixation for electron microscopical, immunocytochemistry 81—82
Fixation, combination 13—14
Fixation, combination, Bouin's 13
Fixation, combination, periodate-lysine-paraformalde-hyde 14
Fixation, combination, Zamboni's 13
Fixation, cross-linking 12—13
Fixation, cross-linking, formalin 12 82
Fixation, cross-linking, glutaraldehyde 12 82
Fixation, freeze-dried tissue 15
Fixation, fresh tissue 14
Fixation, pre-fixed, non-embedded tissue 14—15
Fixation, precipitant 13
Fixation, precipitant, acetone 13 14
Fixation, precipitant, alcohol 13 14
Fluorescent labels 1—2 20 21—23
Fluorescent labels, advantages 21
Fluorescent labels, AMCA 22
Fluorescent labels, counterstains 21
Fluorescent labels, disadvantages 21
Fluorescent labels, fluorescein iv (front cover illustration) 1—2 20 22 36—39 4 15 16)
Fluorescent labels, fluorescein, labelling procedure 21—22
Fluorescent labels, microscopy 105
Fluorescent labels, mountants 106
Fluorescent labels, new fluorophores, CyDyes$^{TM}& 22
Fluorescent labels, new fluorophores, Oregon Green 22
Fluorescent labels, phycoerythrin 22
Fluorescent labels, rhodamine 22
Fluorescent labels, Texas Red iv (front cover illustation) 22
Fluorescent labels, uses 23
Glucose oxidase 2 26—27 121
Glucose oxidase, development 121
Hapten 2 29
Hapten, hapten sandwich method 29
history 1
Immunofluorescence 1—2 20 21—23 36—39 4 15 16)
Immunofluorescence in double staining iv (front cover illustration) 76 77 79 105
Immunofluorescence, counterstains 21
Immunofluorescence, microscopy 105
Immunofluorescence, mountants 106
Immunofluorescence, quantification 97—99
Immunofluorescence, quantification, confocal microscopy 98
Immunofluorescence, quantification, FACS 98—99
Immunofluorescence, quantification, flow cytometry 98—99
Immunofluorescence, uses 23
Immunogold staining for electron microscopy 85—88 132—133
Immunogold staining for electron microscopy, double labelling 87—88
Immunogold staining for light microscopy 27 132—133
Immunogold staining for light microscopy with silver intensification 27 132—133
Immunoperoxidase staining 36 38 see
Immunostaining methods 125—133
Immunostaining methods, immunostaining, all preparations 127—128
Immunostaining methods, initial procedures 125—127
Immunostaining methods, initial procedures, fresh cryostat sections 125—126
Immunostaining methods, initial procedures, paraffin sections 125
Immunostaining methods, initial procedures, pre-fixed frozen sections 126
Immunostaining methods, initial procedures, semithin resin sections 126—127
Immunostaining methods, initial procedures, whole cell preparations 126
Intensification of peroxidase/ DAB/ reaction, during reaction 67
Intensification of peroxidase/ DAB/ reaction, during reaction, cobalt 67 123
Intensification of peroxidase/ DAB/ reaction, during reaction, imidazole 67 123
Intensification of peroxidase/ DAB/ reaction, during reaction, nickel 37 39 14) 67 124—125
Intensification of peroxidase/ DAB/ reaction, during reaction, tyramine signal amplification 67—70
Intensification of peroxidase/ DAB/ reaction, post-reaction 66—70
Intensification of peroxidase/ DAB/ reaction, post-reaction, copper sulphate 37 39 66 122
Intensification of peroxidase/ DAB/ reaction, post-reaction, gold chloride 66 123
Intensification of peroxidase/ DAB/ reaction, post-reaction, silver salts 67
Labelled probes — non- in situ hybridization of nucleic acids 101—102
Labelled probes — non-, lectin histochemistry 101
Labelled probes — non-, receptor localization 100—101
Labelled probes — non-immunocytochemical uses 100—102
labels 1—2 20—29
Labels, biotin 2 29
Labels, colloidal gold 27—28 85—86 128—130 131—133
Labels, enzyme 2 23—27
Labels, fluorescent 21—23
Labels, hapten 2 29
Labels, radioisotope 2 28—29
Methods, APAAP 51 63
Methods, automation 46—47
Methods, avidin-biotin 51—54
Methods, direct (one-step) 47—48
Methods, electron microscopical 81—88 132—133
Methods, general considerations 41—44
Methods, general considerations, antibody diluent 42
Methods, general considerations, antibody dilution 42—43
Methods, general considerations, buffers 41—42
Methods, general considerations, nature of antibodies 44—45
Methods, general considerations, reaction temperature 43—44
Methods, general considerations, reaction time 43
Methods, general considerations, sensitivity 44
Methods, immunogold with silver intensification 27 128—130
Methods, indirect (two-step) 47—49
Methods, multiple immunostaining 73—79 131
Methods, multiple immunostaining, for electron microscopical immuno-labelling 87—88
Methods, PAP 50—51
Methods, procedure for immunostaining 125—133
Microscopy 105—107
Monoclonal antibodies 8—9
Monoclonal antibodies, advantages 8
Monoclonal antibodies, control for non-specific binding 57
Monoclonal antibodies, dilution for immunocytochemistry 10
Monoclonal antibodies, disadvantages 8—9
Monoclonal antibodies, production 8
Multiple staining 73—79 131
Multiple staining, primary antibodies from different species 76—79 87—88 131
Multiple staining, primary antibodies from different species, immunoenzymatic 37 39 78 131
Multiple staining, primary antibodies from different species, immunofluorescence iv (front cover illustration) 76 77 79 105
Multiple staining, primary antibodies from same species 73—77
Nomarski optics 106
Non-specific (background) staining 24—27 29—31 52—61
Non-specific (background) staining, antibody factors 57—59
Non-specific (background) staining, antibody factors, binding (specific) to unknown antigens 57
Non-specific (background) staining, antibody factors, binding to basic proteins 57—58
Non-specific (background) staining, antibody factors, cross-reaction (specific) with related antigens 58—59
Non-specific (background) staining, antibody factors, cross-reaction (specific) with tissue Ig 58
Non-specific (background) staining, remedies 30—31 56 60—61
Non-specific (background) staining, testing 55—58
Non-specific (background) staining, tissue factors 30—31 55—57
Non-specific (background) staining, tissue factors, autofluorescence 31
Non-specific (background) staining, tissue factors, basic proteins 58
Non-specific (background) staining, tissue factors, charged sites 30
Non-specific (background) staining, tissue factors, cross-reactivity (specific) of Ig 58
Non-specific (background) staining, tissue factors, cross-reactivity (specific) of related antigens 58—59
Non-specific (background) staining, tissue factors, endogenous biotin 52—53 112
Non-specific (background) staining, tissue factors, endogenous enzyme 23—27
Non-specific (background) staining, tissue factors, Fc receptors 30
Non-specific (background) staining, tissue factors, hydrophobic reaction 30
Peroxidase 2 24—26
Peroxidase, blocking endogenous enzyme 24 112—113
Peroxidase, development 24—26 117—119
Peroxidase, development, 3-amino-9-ethylcarbazole (AEC) 25 36—39 12) 118—119
Peroxidase, development, 4-chloro-1-naphthol 25—26 119
Peroxidase, development, diaminobenzidine 24—25 66—70 118—119 122—125
Peroxidase, development, phenol tetrazolium 26 119
Peroxidase-anti-peroxidase (PAP), method 50—51 125—128
Peroxidase-anti-peroxidase (PAP), versus ABC 37 39 10)
Photomicrography 106—107
poly-L-lysine, as absorption control 58
poly-L-lysine, as section adhesive 16 110—111
Problems and remedies 56
Quantification 97—100
Quantification, confocal microscopy 37 39 16) 98
Quantification, FACS 98—99
Quantification, flow cytometry 98—99
Quantification, supraoptimal dilution 99—100
Radioactive labels 28—29
Radioimmunoassay, as antibody test 92
Requirements for immunocytochemistry 11—13
Saving failed reactions 65—66
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