Contents
Escherichia coli, Plasmids, and Bacteriophages
Preparation and Analysis of DNA
Enzymatic Manipulation of DNA and RNA
Preparation and Analysis of RNA
Construction of Recombinant DNA Libraries
Screening of Recombinant DNA Libraries
DNA Sequencing
Mutagenesis of Cloned DNA
Introduction of DNA into Mammalian Cells
Analysis of Proteins
Immunology
DNA-Protein Interactions
Yeast
In Situ Hybridization and Immunohistochemistry
The Polymerase Chain Reaction
Protein Expression
Preparation and Analysis of Glycoconjugates
Analysis of Protein Phosphorylation
Informatics for Molecular Biologists
Analysis of Protein Interactions
Chromatin Assembly and Analysis
Nucleic Acid Arrays
Manipulating the Mouse Genome
Generation and Use of Combinatorial Libraries
Discovery and Analysis of Differentially Expressed Genes in Single Cells and Cell
Populations
Gene Silencing
RNA-Protein Interactions
Mammalian Cell Culture
Mouse Phenotyping
Appendicies