This book constitutes the refereed proceedings of the 7th European Conference on Evolutionary Computation, Machine Learning and Data Mining in Bioinformatics, EvoBIO 2010, held in Istanbul, Turkey, in April 2010 co-located with the Evo* 2010 events. This 15 revised full papers were carefully reviewed and selected from 40 submissions. EvoBIO is the premiere European event for those interested in the interface between evolutionary computation, machine learning, data mining, bioinformatics, and computational biology. Topics addressed by the papers include biomarker discovery, cell simulation and modeling, ecological modeling, fluxomics, gene networks, biotechnology, metabolomics, microarray analysis, phylogenetics, protein interactions, proteomics, sequence analysis and alignment, and systems biology.