This book constitutes the refereed proceedings of the 13th International Symposium on Recent Advances in Intrusion Detection, RAID 2010, held in Ottawa, Canada, in September 2010. The 24 revised full papers presented together with 15 revised poster papers were carefully reviewed and selected from 102 submissions. The papers are organized in topical sections on network protection, high performance, malware detection and defence, evaluation, forensics, anomaly detection as well as web security.