This volume opens with an overview of the basic theory and general methodology of HPLC, with particular reference to the key separation parameters that can be manipulated to achieve high resolution. Each of the commonly used HPLC techniques are covered in Chapters 2–9, whereas methods for capillary to large-scale preparative isolation are described in Chapters 10–
15. Chapters 16–27 provide those already experienced in HPLC with a number of specific applications, as in case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology.
These applications include proteolytic mapping, posttranslational modifications, neuropeptide processing, glycopeptides and glycoproteins, MHC-binding peptides, toxins/venoms, membrane proteins, antibodies, combinatorial and proteome analysis, and enzymatic activity. HPLC of Peptides and Proteins: Methods and Protocols will be a valuable resource for a wide range of scientists, including biochemists, molecular biologists, pharmacologists, and microbiologists, who work with peptides and/or proteins in both academic and biotechnology laboratories.