Regenerative Medicine is devoted to replacing diseased cells, tissues, or organs, or repairing tissues in vivo by augmenting natural or inducing latent regenerative processes. Underlying these goals is the manipulation – both expansion and directed differentiation – of stem cells, which are the primary source of de novo tissue regeneration and maintenance of organ homeostasis. In this book, Stem Cells in Regenerative Medicine, we aim to provide biomedical researchers, clinicians and biomedical engineers an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, starting from the isolation and culture of stem cells to their actual use in vivo. In this first edition, we have attempted to compile a foundation of protocols which can be refined in subsequent publications. We hope that this series of protocols will contribute to the definition of standardized procedures for the manipulation of somatic and embryonic stem cells in research and clinical applications.