This book constitutes the refereed proceedings of the 14th Australasian Conference on Information Security and Privacy, ACISP 2009, held in Brisbane, Australia, during July 1-3, 2009. The 29 revised full papers presented together with two invited talks were carefully reviewed and selected from 106 submissions. The papers are organized in topical sections on network security, symmetric key encryption, hash functions, public key cryptography, protocols, and implementation.