The International Congress on Mathematical Physics is the flagship conference in this exciting field. Convening every three years, it gives a survey on the progress achieved in all branches of mathematical physics. It also provides a superb platform to discuss challenges and new ideas. The present volume collects material from the XVIth ICMP which was held in Prague, August 2009, and features most of the plenary lectures and invited lectures in topical sessions as well as information on other parts of the congress program. This volume provides a broad coverage of the field of mathematical physics, from dominantly mathematical subjects to particle physics, condensed matter, and application of mathematical physics methods in various areas such as astrophysics and ecology, amongst others.