New platforms of ultrasensitive analysis of biomolecules and single living cells using multiplexing, single nanoparticle sensing, nano-fluidics, and single-molecule detection are advancing every scientific discipline at an unprecedented pace. With chapters written by a diverse group of scientists working in the forefront of ultrasensitive bioanalysis, this book provides an overview of the current status and an in-depth understanding of the objectives and future research directions of ultrasensitive bioanalysis. Spanning a wide spectrum of new research approaches, this book:

• Introduces new theories, ideas, methodologies, technologies, and applications of ultrasensitive bioanalysis in a wide variety of research fields
• Includes background, fundamentals, and descriptions of instrumentation and techniques behind every experimental design and approach to help readers explore the promising applications of new tools
• Covers single molecule detection (SMD), single living cell analysis, multi-functional nanoparticle probes, miniaturization, multiplexing, quantitative and qualitative analysis of metal ions and small molecules, and more
• Discusses techniques such as single molecule microscope and spectroscopy, single nanoparticle optics, single nanoparticle sensors, micro- and nano-fluidics, microarray detection, ultramicroelectrodes, electrochemiluminescence, mass spectrometry, and more

This book will be a useful resource and an inspiration for scientists and graduate and undergraduate students in a wide variety of research fields, including chemistry, biology, biomedical science and engineering, and materials science and engineering.Content:
Chapter 1 Is One Enough? (pages 1–27): Andrew C. Beveridge, James H. Jett and Richard A. Keller
Chapter 2 Dissecting Cellular Activity from Single Genes to Single mRNAs (pages 29–39): Xavier Darzacq, Robert H. Singer and Yaron Shav?Tal
Chapter 3 Probing Membrane Transport of Single Live Cells Using Single?Molecule Detection and Single Nanoparticle Assay (pages 41–70): Xiao?Hong Nancy Xu, Yujun Song and Prakash Nallathamby
Chapter 4 Nanoparticle Probes for Ultrasensitive Biological Detection and Imaging (pages 71–89): Amit Agrawal, Tushar Sathe and Shuming Nie
Chapter 5 Tailoring Nanoparticles for the Recognition of Biomacromolecule Surfaces (pages 91–117): Mrinmoy De, Rochelle R. Arvizo, Ayush Verma and Vincent M. Rotello
Chapter 6 Nanoscale Chemical Analysis of Individual Subcellular Compartments (pages 119–140): Gina S. Fiorini and Daniel T. Chiu
Chapter 7 Ultra Sensitive Time?Resolved Near?IR Fluorescence for Multiplexed Bioanalysis (pages 141–168): Li Zhu and Steven A. Soper
Chapter 8 Ultrasensitive Microarray Detection of DNA using Enzymatically Amplified SPR Imaging (pages 169–194): Hye Jin Lee, Alastair W. Wark and Robert M. Corn
Chapter 9 Ultrasensitive Analysis of Metal Ions and Small Molecules in Living Cells (pages 195–214): Richard B. Thompson
Chapter 10 Electrochemistry Inside and Outside Single Nerve Cells (pages 215–234): Daniel J. Eves and Andrew G. Ewing
Chapter 11 Electrochemiluminescence Detection in Bioanalysis (pages 235–267): Xiao?Hong Nancy Xu and Yanbing Zu
Chapter 12 Single?Cell Measurements with Mass Spectrometry (pages 269–293): Eric B. Monroe, John C. Jurchen, Stanislav S. Rubakhin and Jonathan V. Sweedler
Chapter 13 Outlooks of Ultrasensitive Detection in Bioanalysis (pages 295–299): Xiao?Hong Nancy Xu