Overview of Antibody Use in Immunocytochemistry.
Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration.
Purification of Antibodies Using Ion-Exchange Chromatography
Purification of Antibodies Using Affinity Chromatography
Purification of Antibodies Using Protein A-Sepharose and FPLC
Conjugation of Fluorochromes to Antibodies
Biotinylation of Antibodies
Overview of Cell Fixatives and Permeabillization
Preparation of Frozen Sections for Analysis
Processing of Cel-Touch Preparation
Processing of Cytological Specimens
Processing of Tissue Culture Cells
Processing of Tissue Specimens
Overview of Fluorophores
Direct Immunofluorescent Labeling of Cells
Fluorescence Labeling of Surface Antigens on Attached or Suspended Tissue-Culture Cells
Fluorescence Labeling of Intracellular Antigens in Attached or Suspended Tissue-Culture Cells
Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts
Overview of Antigen Detection Through Enzymatic Activity
The Peroxidase Antiperoxidase (PAP) Method
The Avidin Biotin Complex (ABC) Method
Labeling Avidin Binding (LAB) Method
Avidin Biotin Labeling of Cellular Antigens in Cryostat Sectioned Tissue
ELISA on Attachment Dependent or Suspension Grown Cells
Use of Immunogold with Silver Enhancement
Overview of Flow Cytometry and Fluorescent Probes for Cytometry
Tissue Disaggregation
Indirect Immunofluorescent Labeling of Viable Cells
Indirect Immunofluorescent Labeling of Fixed Cells
Fluorescent Labeling of DNA
Deparaffinization and Processing of Pathologic Material
Assay for Phagocytosis
Assay for Filamentous Actin
Assay for Chemoattractant Binding
Assay for Oxidative Metabolism
Fixation and Embedding
Preparation of Colloidal Gold
Conjugation of Colloidal Gold to Proteins
Colloidal Gold/Streptavidin Methods
Pre-Embedding Labeling Methods
Postembedding Labeling Methods
Overview of Fluorescence Photomicrography
Overview of Fluorescence Analysis with the Confocal Microscope
Overview of Laser Microbeam Applications as Related to Antibody Targeting
Nucleic Acid Immunocytochemistry
Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes
Overview of Automated Immunostainers
Overview of Immunocytochemical Approaches to the Differential Diagnosis of Tumors