The book constitutes the refereed proceedings of the International Workshop on Distributed, High-Performance and Grid Computing in Computational Biology, GCCB 2006, held in Eilat, Israel in January 2007 in conjunction with the 5th European Conference on Computational Biology, ECCB 2006. The 13 revised full papers presented were carefully reviewed and selected from many high quality submissions. All current issues of computational biology, bioinformatics and life science are addressed concerning distributed, high-performance and grid computing technologies in the context of computational biology and bioinformatics. The papers are organized in topical sections on sequence analysis, grids for screening and property prediction, data management, and collaborative environments.